Comparative proteomic map among vanA-containing Enterococcus isolated from yellow-legged gulls

DOI: 10.5584/jiomics.v2i1.86

Authors

  • Hajer Radhouani Institute for Biotechnology and Bioengineering, Center of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  • Patrícia Poeta Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  • Luís Pinto Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  • Ricardo Monteiro Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  • Júlio Nunes-Miranda Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro; Vila Real, Portugal
  • Susana Correia Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro; Vila Real, Portugal
  • Carlos Carvalho Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro; Vila Real, Portugal
  • Jorge Rodrigues Veterinary Science Department, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  • María Lopez Biochemistry and Molecular Biology Area, University of La Rioja, Logroño, Spain
  • Carmen Torres Biochemistry and Molecular Biology Area, University of La Rioja, Logroño, Spain
  • Rui Vitorino Chemistry Department, University of Aveiro, Aveiro, Portugal
  • Pedro Domingues Chemistry Department, University of Aveiro, Aveiro, Portugal
  • Gilberto Igrejas Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal

Abstract

 

The increase of VRE, therefore, represents an urgent threat for patient care and creates a reservoir of mobile resistance genes for other, more virulent pathogens. The existence of VRE in different ecologic niches complicates the understanding of its epidemiology. The aim of the present study was to study the proteome of 2 vanA strains recovered from seagull faecal samples. The vanA E. durans and vanA E. faecium isolates presented different genomic patterns: tet(M)-tet(L)-erm(B) and tet(M)-tet(L)-erm(B)-hyl, respectively. A total of 123 spots were excised from two-dimensional gel electrophoresis (2-DE) gel of vanA E. durans SG 2 strain, and 16 were successfully identified by using MS, representing 42 different proteins. For the vanA E. faecium SG 50 strain, 93 spots were excised from the 2-DE gel and 23 were identified, representing 47 different proteins. The vancomycin/teicoplanin A-type resistance protein vanA in vanA E. durans SG 2 strain was present in two different spots. The identified proteins have shown diverse functional activities including glycolysis, conjugation, translation, protein biosynthesis, among others. This work reports the impact of proteomics on knowledge of vanA enterococci strains and will be helpful to further understand the antibiotic-resistant mechanism.

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Published

2012-05-31

Issue

Vol. 2 No. 1 (2012): Vol 2, No 1 (2012)

Section

Articles