Journal of Integrated OMICS

Vol. 14 No. 1 (2024)

José Luis Capelo — 2024-04-15

Navigating the molecular diversity of SARS-CoV-2: early pandemic insights from comparative phylogenetic analysis

Chahinez Triqui — 2024-04-15

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in December 2019 precipitated the onset of the COVID-19 pandemic, which swiftly spread across more than 214 countries and territories, posing a significant global health crisis. In response, laboratories worldwide have embarked on extensive efforts to characterize the genomic landscape of the virus, employing a myriad of sophisticated genomic analysis techniques. This study endeavors to undertake a comprehensive exploration into the genetic diversity, geographical distribution, and virulence determinants of SARS-CoV-2 clades across 11 diverse countries, employing advanced computational biology methodologies. Leveraging molecular data sourced from prominent international databases, the analysis aims to unravel the intricate phylogenetic relationships and mutational dynamics exhibited by various viral strains circulating worldwide. The findings of this investigation promise to yield invaluable insights into the evolutionary trajectory of SARS-CoV-2, shedding light on potential therapeutic targets and informing strategies for mitigating the impact of the ongoing pandemic on global public health. Results highlight significant genetic diversity among SARS-CoV-2 strains across different countries, with phylogenetic analysis revealing distinct subclass groupings within each country. A manual comparison of sequences identified numerous mutations, with certain mutations associated with increased virulence. Comparison of clade G and clade O sequences revealed differences in mutation profiles, suggesting potential links to virulence and transmissibility. These findings underscore the dynamic nature of SARS-CoV-2 evolution and the importance of monitoring genetic changes for public health interventions.

DTT protein equalization and Tryptophan protein quantification as a powerful tool in analytical proteomics.

Inês F. Domingos — 2024-04-15

Assessing total protein levels in biological samples is a common procedure in biochemistry and molecular biology. In this study, we compare tryptophan fluorescence (WF) with Bradford and BCA assays to determine total protein in serum samples. Our results indicate that tryptophan fluorescence spectrometry is an efficient, sensitive, and straightforward technique for quantifying proteins in serum. We observed minimal variation between the three methods: BCA de one with the lowers LOD and LOQ. The tryptophan method offers the possibility of reusing the intact sample that does not need colourimetric reagents for quantification. Consequently, free tryptophan serves as a reliable universal standard. This assay can be performed using a conventional fluorescence spectrometer with cuvettes or in a 96-well plate format with a plate reader. The method was successfully used as proof of concept, using serum from patients diagnosed with myeloma and serum from healthy donors.