THE ROLE OF METALLOTHIONEINS IN MAINTENANCE OF ZINC HOMEOSTASIS AND REDOX STATE IN ERYTHROCYTES OF CARDIOLOGIC PATIENTS WITH METABOLIC SYNDROME
The comparative analysis of the intracellular labile zinc pool, level of the reduced glutathione and cystein-rich proteins metallothioneins taking into account the viability of erythrocytes of patients with diagnosed components of the metabolic syndrome (presence of an arterial hypertension and diabetes mellitus type 2) with coronary heart disease is conducted. Fluorescent probes (FluoZin-3-AM and Calcein-AM), Ellman’s reagent and monoclonal antibody (anti-metallothionein UC1MT) have used for this. The received results demonstrate an important role of zinc homeostasis (decreasing of cytosolic Zn2+ level) in the etiopathogenesis of diabetes mellitus type 2 and in the development of metabolic syndrome in general. Increasing of metallothioneins expression and significant decrease of the reduced glutathione level in erythrocytes of testing patients indicate about functioning of the first as an additional antioxidants in human erythrocytes defense system under this pathology.