THIOCHROME ACTIVATES DNA-POLYMERASE I

DOI: 10.5584/jiomics.v9i1.261

Abstract

We studied the effect of this metabolite on the activity of DNA polymerase I isolated from the liver of white rats.Established groups in the molecule of DNA polymerase are involved in the interaction thiochrome and responsible for the activation of this enzyme.This article shows the possibility of the influence of the most important metabolite –thiochrome on DNA polymerase I. Role of carboxyl groups of the enzyme and hydrophobic and hydrogen bonds in it in the realization of the activating action of thiochrome on the given enzyme has been studied. The influence of thiochrome on the activity of DNA polymerase was researched. It was found that thiochrome is capable of activating this enzyme through interaction with two sites of the enzyme containing amino acids with hydrophobic radicals.