A DIGE proteomic analysis of wheat flag leaf treated with TERRA-SORB® foliar, a free amino acid high content biostimulant

DOI: 10.5584/jiomics.v6i1.188

Authors

  • Maria Jose Martinez Esteso Grupo de Proteómica y Genómica Funcional de Plantas. Dept. Agroquímica y Bioquímica. Universidad de Alicante, Ctra. de San Vicente del Raspeig, s/n, 03690 Alicante, Spain
  • Maite Vilella-Antón Grupo de Proteómica y Genómica Funcional de Plantas. Dept. Agroquímica y Bioquímica. Universidad de Alicante, Ctra. de San Vicente del Raspeig, s/n, 03690 Alicante, Spain
  • Susana Sellés-Marchart Unidad de Genómica y Proteómica, SSTTI, Universidad de Alicante, Ctra. de San Vicente del Raspeig, s/n, 03690 Alicante, Spain
  • Ascensión Martínez-Márquez Grupo de Proteómica y Genómica Funcional de Plantas. Dept. Agroquímica y Bioquímica. Universidad de Alicante, Ctra. de San Vicente del Raspeig, s/n, 03690 Alicante, Spain
  • Anna Botta-Catala Departamento de I+D Fisiología Vegetal, BIOIBERICA, S.A., Plaza Francesc Maciá 08029 Barcelona, Spain
  • Rafael Piñol-Dastis Departamento de I+D Fisiología Vegetal, BIOIBERICA, S.A., Plaza Francesc Maciá 08029 Barcelona, Spain
  • Roque Bru-Martinez IMEM Ramón Margalef, Grupo de Proteómica y Genómica Funcional de Plantas. Dept. Agroquímica y Bioquímica. Universidad de Alicante, Ctra. de San Vicente del Raspeig, s/n, 03690 Alicante, Spain

Abstract

The flag leaf is the most important source of carbohydrate during wheat kernel filling. Around a 75% of all sugars stored in the kernel come from carbon fixed by this leaf. Terra-Sorb® foliar is an L-α-amino acid-based product from enzymatic hydrolysis for foliar application with a high ratio of free to total amino acids. Previous agronomical studies carried out on grassy, horticultural and tree crops have shown that the application of Terra-Sorb® increases photosynthetic plant activity and chlorophyll content, promotes rapid recovery from stress and improves fruit set.

In this work, we have undertaken a proteomic approach to explore molecular mechanisms potentially involved in the stimulating effect of Terra-Sorb® Foliar on wheat yield when applied in commercial fields. Wheat plants at the flag leaf stage were treated, and a DIGE approach was used to compare the proteomes of treated vs. control plants in four biological replicates.

Thirty-seven protein spots were found to change in abundance (ANOVA p

Published

2016-06-30